Coding

Part:BBa_K4482000:Design

Designed by: Mustafo Mustafokulov   Group: iGEM22_NYC_Empire_State   (2022-08-25)


Human FGFR2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2276
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2276
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2276
    Illegal BglII site found at 2084
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2276
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2276
    Illegal NgoMIV site found at 786
    Illegal NgoMIV site found at 1236
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1386
    Illegal BsaI.rc site found at 2238
    Illegal SapI.rc site found at 106


Design Notes

The coding sequence was extracted from the original plasmid using PCR and inserted into a new plasmid backbone to form a fusion protein with eGFP.


Source

The source of this part is an AddGene plasmid with code 116741.

References